Somatostatin-Expressing Neurons Regulate Sleep Deprivation and Recovery

TL;DR

This study identifies somatostatin-expressing neurons as key players in sleep deprivation and recovery through gene expression analysis in mice.

Contribution

A data-driven transcriptomic approach reveals the role of somatostatin-expressing neurons in sleep deprivation and recovery.

Findings

Selected gene sets were enriched in various brain regions, indicating biological relevance.

Somatostatin-expressing neuronal clusters were among the most highly enriched in the analysis.

Findings were validated using AlphaGenome with the selected genes.

Abstract

Background/Objectives We re-analyzed publicly available gene expression profiles from the male mouse cortex under conditions of sleep deprivation (SD) using tensor decomposition-based unsupervised feature extraction, originally proposed by one of the authors in 2017. Methods We focused on two distinct expression patterns: genes whose levels were altered in SD and failed to normalize during recovery sleep (RS), and genes that overshot normal levels during RS. This selection excluded the expected “altered in SD and recovered in RS” pattern, which was not significantly observed. These two gene sets showed substantial overlap but were still distinct from each other. Results The analysis revealed that the selected gene sets were enriched in various brain regions as evidenced through clustering in the Allen Brain Atlas. This suggests that the successful selection identified biologically meaningful genes. Furthermore, somatostatin (Sst)-expressing neuronal clusters were among the most highly enriched. Conclusions Given that sst is already implicated in SD and RS, our fully data-driven transcriptomic analysis successfully identified the activity of sst during SD and RS. These findings reveal that Sst-expressing neurons may play a key role in SD. These results were further validated using AlphaGenome by uploading the selected genes to it.

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