# Investigating the Anti-Inflammatory Activity of Juglans regia Fresh Fruit Extract

## TL;DR

This study explores the anti-inflammatory effects of a fresh fruit extract from Juglans regia in animal models and through computational analysis.

## Contribution

The study demonstrates the anti-inflammatory potential of Juglans regia extract in both acute and chronic inflammation models.

## Key findings

- The extract significantly reduced licking time in the formalin test, particularly in the late phase.
- Quercetin showed the best docking score for AChE, while neochlorogenic acid and ellagic acid had strong scores for BChE.
- Abscisic acid exhibited high binding affinity for the glucocorticoid receptor.

## Abstract

Numerous research works have tried to evaluate the correlation between inflammation and the onset of prostate cancer. Given the in vitro antioxidant power and the anti-proliferative effects on human prostate cancer cells shown by a Juglans regia L. fresh fruit extract, the aim of this work was the evaluation of its potential in the acute and chronic inflammatory states in vivo, revealing a strong anti-inflammatory activity. In the zymosan-induced edema formation assay, a light and non-significant edema reduction was shown. On the contrary, in the zymosan-induced thermal hyperalgesia assay, the reversion of hyperalgesia after the extract administration was determined. Moreover, in the formalin test, the extract caused a significant decrease in the licking time caused by the aldehyde, especially in the late phase. In silico, quercetin showed the best fit into the enzymatic pocket of AChE (docking score: −11.306 Kcal/mol). Neochlorogenic acid and ellagic acid gave the best docking scores on BChE (−10.292 Kcal/mol and −10.054 Kcal/mol, respectively). Abscisic acid showed a high binding affinity for the glucocorticoid receptor. Finally, quercetin and abscisic acid were quantified to complete the data by HPLC-DAD, giving 0.246 ± 0.003 mg/g of dried extract and 0.036 ± 0.004 mg/g of dried extract, respectively.