# Integrated Analysis of Behavioral and Physiological Effects of Nano-Sized Carboxylated Polystyrene Particles on Daphnia magna Neonates and Adults: A Video Tracking-Based Improvement of Acute Toxicity Assay

## TL;DR

The paper introduces a smartphone-based platform to detect nanoplastic toxicity in Daphnia magna by analyzing heart rate and behavior, offering a more sensitive and cost-effective method for environmental monitoring.

## Contribution

The novel contribution is integrating behavioral and physiological analysis using a smartphone platform to detect early nanoplastic toxicity effects in Daphnia magna.

## Key findings

- Short-term exposure to carboxylated polystyrene nanoparticles significantly affected heart rate in Daphnia magna neonates and adults.
- Behavioral changes were observed in Daphnia magna exposed to 40 nm particles but not 200 nm particles.
- The smartphone platform effectively integrates real-time behavioral and physiological data for toxicity assessment.

## Abstract

Nanoplastics pose significant environmental and public health risks, prompting the need for sensitive, cost-effective, and rapid assays for ecotoxicity assessment. The present work proposes the use of a portable smartphone-based platform to enhance traditional Daphnia magna acute toxicity assays by integrating behavior analysis and heart rate measurements. The aim is to improve sensitivity in detecting toxic effects of nanoplastics. In particular, the study focused on nano-sized carboxylated polystyrene (PS) nanoparticles. Two variability factors that could influence biological effects of nanoplastics, the particle size and the age of the organisms, were considered. Results demonstrated that the application of the proposed integrated approach allowed the detection of early subtle effects such as a significant impact on the heart rate and behavior of Daphnia magna under short-term exposure to PS carboxylated nanoparticles. In particular, a stimulation of heart rate was observed for both neonates and adults either for 40 nm or 200 nm particles after 48 h exposure, presumably attributable to an interference of carboxylated PS NPs with adrenergic-type receptors. Behavioral alterations were detectable for 40 nm particles but not for 200 nm ones consisting of a decrease in velocity and alterations of trajectories. Obtained results demonstrated the suitability of the proposed smartphone platform for friendly and real-time integration of behavioral analysis with physiological outcome measurements during acute exposure of Daphnia magna to nano-sized carboxylated PS NPs, expanding the sensitivity of the traditional acute toxicity tests. It offers a novel, cost-effective, and field-applicable method for environmental monitoring of nanoparticle toxicity and impact.