# Secondary metabolism of Microcystis: current understanding and recent advances in unlocking genomic and chemical diversity

## TL;DR

This review explores the diverse secondary metabolites of Microcystis, highlighting their ecological roles and potential for biotechnology, while emphasizing the need for further research.

## Contribution

The paper synthesizes recent advances in understanding the genomic and chemical diversity of Microcystis secondary metabolism.

## Key findings

- Microcystis strains show significant variation in biosynthetic gene clusters (BGCs) and their expression.
- Many BGCs and metabolites remain uncharacterized, limiting knowledge of their functions and bioactivity.
- High-throughput multiomic approaches are proposed to link gene clusters with their metabolites.

## Abstract

The cyanobacterial genus Microcystis is globally distributed and known for its ability to produce microcystins, a structurally diverse group of cyanotoxins. However, the biosynthetic capacity of Microcystis is vast; its diverse genomes contain a variety of biosynthetic gene clusters (BGCs) encoding the synthesis of metabolites that may be toxic, have important ecological function, or have applications for biotechnology or drug discovery. Recent studies illustrate that these BGCs vary significantly across Microcystis strains, can be highly expressed in environmental conditions, and may play key roles in cellular physiology, grazer deterrence, and microbial interactions. However, many of these BGCs and metabolites remain poorly characterized or completely uncharacterized, having been identified only through genome sequencing or mass spectrometry, respectively, leaving no knowledge of their structure, bioactivity, or physiological or ecological functions. Here, we synthesize the current body of knowledge regarding the secondary metabolism of Microcystis in terms of genetic and chemical diversity, potential drivers of synthesis, and physiological and ecological functions. This review highlights the need for further research to characterize the largely unexplored genetic and chemical diversity of Microcystis in communities in the environment and discusses the challenges and opportunities of integrating high-throughput multiomic approaches to link uncharacterized gene clusters with their corresponding metabolites. Microcystis will continue to be a rich source for secondary metabolite research as its genetic and chemical potential likely plays a critical role in the persistence and observed dynamics of harmful algal blooms and may harbor uncharacterized toxins and metabolites.