# Synthesis and Characterization of Ibuprofen–TiO2 Functionalized PCL Biomembranes as Candidate Materials for Wound Dressing Applications

## TL;DR

This paper explores the creation of wound dressing membranes using PCL, ibuprofen, and titanium dioxide to improve healing and prevent infection.

## Contribution

The study introduces a new PCL-based biomembrane functionalized with TiO2 and ibuprofen for wound dressing applications.

## Key findings

- The membranes showed increased surface roughness and molecular stability during fabrication.
- In vitro tests showed good biocompatibility with human fetal osteoblasts.
- No cytotoxicity was observed in any of the membrane groups.

## Abstract

Wound dressing coverages (WDC) play a key role in protecting skin lesions and preventing infection. Polymeric membranes have been widely explored as WDC due to their ability to incorporate bioactive agents, including antimicrobial nanoparticles and non-steroidal anti-inflammatory drugs (NSAIDs). In this study, polycaprolactone (PCL)-based membranes functionalized with titanium dioxide nanoparticles (TiO2 NPs) and ibuprofen (IBP) were fabricated using a film manufacturing approach, and their structural and biocompatibility profiles were evaluated. The membranes were characterized by SEM, FTIR and XPS. Bands at 1725 cm−1, 2950 cm−1, 2955 cm−1, 2865 cm−1 and 510 cm−1 proved molecular stability of reagents during manufacture. In SEM, the control shows the flattest surface, while the PCL-IBP and PCL-IBP-TiO2 NPs groups had increased rugosity. In vitro biocompatibility was evaluated using human fetal osteoblasts (hFOB). On day 3, the cell adhesion response of hFOB seeded in PCL-IBP and PCL-IBP-TiO2 NPs groups showed the biggest absorbances (p = 0.0014 and p = 0.0491, respectively). On day 7 PCL-IBP group had lower lectin binding than the control (p = 0.007) and the PCL-IBP-TiO2 NPs (p = 0.015) membranes, but no evidence of cytotoxicity was observed in any group. Furthermore, the Live/Dead test adds more biocompatibility evidence to conveniently discriminate between live and dead cells. The PCL polymeric membrane elaborated in this study may confer antiseptic, analgesic and anti-inflammatory properties, making these membranes ideal for skin lesions.