# Effect of Irrigation Activation Techniques on Periapical Organic Tissue Dissolution in Simulated Immature Teeth: An Ex Vivo Study

## TL;DR

This study compares different irrigation techniques for dissolving tissue around the root tips of immature teeth, finding that some methods are more effective than others.

## Contribution

The study introduces a novel ex vivo model to evaluate irrigation techniques for periapical tissue dissolution in immature teeth.

## Key findings

- Ultrasonic irrigation (UI) showed significantly less tissue dissolution compared to other methods.
- Standard needle irrigation (SNI), PIPS, and SWEEPS performed similarly in dissolving tissue.
- All irrigation methods resulted in some degree of tissue loss.

## Abstract

Background/Objectives: Effective removal of organic tissue extruded beyond the apex is crucial in regenerative endodontics, particularly in teeth with immature apices; therefore, this study aims to compare the efficacy of standard needle irrigation (SNI), ultrasonic irrigation (UI), photon-induced photoacoustic streaming (PIPS), and shock wave-enhanced emission photoacoustic streaming (SWEEPS) techniques in dissolving periapical tissue in a simulated model. Methods: Sixty single-rooted human premolars and sixty bovine palatal mucosa specimens were used. A custom model was created by placing mucosal tissue in contact with the apical area. Specimens were divided into four groups (n = 15) according to the irrigation method: SNI, UI, PIPS, and SWEEPS. Each canal received 15 mL of 2% NaOCl. Tissue samples were weighed before and after treatment. One-way ANOVA and Tukey’s post hoc test were used for statistical analysis (p < 0.05). Results: UI showed significantly less tissue dissolution than the other methods (p < 0.05). SNI, PIPS, and SWEEPS showed no significant differences (p > 0.05). Conclusions: All methods led to tissue loss, but UI was significantly less effective. SNI, PIPS, and SWEEPS performed similarly.