# Production of Carbon Sources Through Anaerobic Fermentation Using the Liquid Phase of Food Waste Three-Phase Separation: Influencing Factors and Microbial Community Structure

## TL;DR

This study shows that fermenting the liquid part of food waste can create a useful carbon source for sewage treatment.

## Contribution

The study identifies optimal fermentation conditions and evaluates the effectiveness of the resulting carbon source for denitrification.

## Key findings

- Mesophilic fermentation at 35 °C with an initial alkaline pH maximized VFA yield and acetic acid fraction.
- Fermented liquid from food waste achieved near-complete nitrate removal in denitrification tests.
- Acidogenic bacteria increased while methanogen archaea decreased under optimal fermentation conditions.

## Abstract

The urgent need for effective food waste management, coupled with the scarcity of carbon sources for sewage treatment, highlights the potential of producing carbon sources from food waste as a mutually beneficial solution. This study investigated the production of carbon sources through anaerobic fermentation using the liquid phase of food waste three-phase separation. Compared with previous studies using raw food waste or mixed substrates, the liquid phase derived from three-phase separation is richer in soluble organic matter and has been pre-heated (80 °C), which facilitates subsequent fermentation and offers easier integration into existing food waste treatment plants. A series of lab-scale batch fermentation experiments were carried out at different temperatures, including ambient, mesophilic, and thermophilic conditions, as well as varying initial pH levels (uncontrolled, neutral, and alkaline). The experimental results indicated that optimal production parameters involve a 4-day mesophilic fermentation at 35 °C with an initial alkaline pH, which increased the total VFAs yield by 252.5% to 40.26 g/L and raised the acetic acid fraction to 45.5% of total VFAs. Under these conditions, there was an observed increase in the relative abundance of acidogenic bacteria and a decrease in that of methanogen archaea. Furthermore, the denitrification performance of the produced carbon source was evaluated in short-term tests, and near-complete nitrate removal was achieved within approximately 2 h. These findings suggest the fermented liquid phase of food waste is a promising partial substitute for conventional external carbon sources.