# Seasonal Variation in PM2.5 Composition Modulates Oxidative Stress and Neutrophilic Inflammation with Involvement of TLR4 Signaling

## TL;DR

This study shows that seasonal changes in PM2.5 composition affect lung inflammation and oxidative stress, with winter PM2.5 being more harmful due to specific components.

## Contribution

The study demonstrates that seasonal PM2.5 composition, not just mass, drives distinct biological effects, with winter PM2.5 preferentially activating IL-1α pathways.

## Key findings

- Winter PM2.5 enriched in PAHs and minerals increases ROS and neutrophil infiltration.
- Mineral components in winter PM2.5 strongly correlate with IL-1α production.
- TLR4 signaling partially mediates PM2.5-induced pulmonary inflammation.

## Abstract

Seasonal fluctuations in the chemical composition of fine particulate matter (PM2.5) are known to influence its toxicological properties; however, their integrated biological effects remain incompletely understood. In this study, PM2.5 was continuously collected over two consecutive years at a single urban site in Japan and classified by season. The samples were comprehensively characterized for ionic species, metals, carbonaceous fractions, and polycyclic aromatic hydrocarbons (PAHs), and their pulmonary effects were evaluated in vivo following intratracheal administration in mice. Seasonal PM2.5 exhibited pronounced compositional differences, with higher levels of secondary inorganic aerosol components in summer and enrichment of PAHs and mineral-associated components in winter. These seasonal differences translated into distinct biological responses. Reactive oxygen species (ROS) production (1.6–2.7-fold increase) and bronchoalveolar lavage (BAL) neutrophil infiltration were strongly associated with PAH-rich PM2.5, whereas interleukin-1α (IL-1α) showed robust positive correlations with mineral components, including K+, Ca2+, and Mg2+, which were predominantly enriched in winter PM2.5. In contrast, secondary inorganic aerosol species displayed a limited capacity to induce IL-1α. Compared with summer samples, winter PM2.5 induced significantly higher levels of ROS production and IL-1α (approximately 1.5–2.6-fold increase). Using TLR2- and TLR4-deficient mice, we further demonstrated that PM2.5-induced increases in BAL cell counts, ROS, IL-6, and TNF-α were partially attenuated in TLR4 knockout mice, indicating a contributory but not exclusive role for TLR4 signaling in PM2.5-driven pulmonary inflammation. Collectively, these findings demonstrate that seasonal variations in PM2.5 composition, not particle mass alone, critically shape oxidative stress and innate immune responses in the lungs. In particular, winter PM2.5 enriched in mineral-associated components preferentially activates IL-1α-mediated alarmin pathways, underscoring the importance of the particle composition in determining seasonal air pollution toxicity.