# Effect of Dietary Supplementation with Rumen-Protected GABA (γ-Aminobutyric Acid) on Milk Productivity and Blood Profiles of Dairy Cattle Under Heat Stress Conditions

## TL;DR

This study shows that adding rumen-protected GABA to the diet of heat-stressed dairy cows can help maintain milk production and improve milk quality.

## Contribution

The study introduces rumen-protected GABA as a novel supplement to mitigate heat stress effects in dairy cows.

## Key findings

- GABA supplementation increased milk fat and lactose content in heat-stressed cows.
- Higher GABA doses tended to reduce heat-related milk yield declines.
- GABA improved cow activity and reduced rest and rumination times.

## Abstract

This study evaluated the effects of rumen-protected γ-aminobutyric acid (GABA) supplementation on milk productivity, blood metabolites, and behavior in heat-stressed Holstein cows. Eighteen cows were assigned to three groups—control, 3 g/d GABA, and 6 g/d GABA—and fed a basal TMR diet over 56 days in summer. Though overall milk production did not differ significantly, higher GABA supplementation tended to lessen heat-related yield declines. At week 8, both GABA groups showed ~4% higher yields versus baseline, while the control did not improve. Significant increases in milk fat (p = 0.036) and lactose (p = 0.017) were observed in GABA-supplemented cows. These findings suggest that rumen-protected GABA helps mitigate heat stress impacts and supports milk yield and composition in Holstein cows during hot periods.

This study was conducted to evaluate the effect of rumen-protected γ-aminobutyric acid (GABA) supplementation on milk productivity of lactating Holstein cows. Eighteen Holstein dairy cows (mean parity, 2.2 ± 1.0 year; mean milk yield, 34.3 ± 5.5 kg) were selected in a commercial dairy farm for the experiment. The experiment was conducted from 17 July 2024 to 11 September 2024 (56 days). Generally, THI 72 is set as a threshold since the productivity of Holstein cows starts to decrease. Animals were exposed to heat stress conditions (THI ≥ 72) during the experimental period. The basal diet was fed as a total mixed ration (TMR), and GABA was top-dressed onto the TMR. The treatments were basal diet (Control), basal diet supplemented with rumen-protected GABA 3 g/d (Treatment 1), and basal diet supplemented with rumen-protected GABA 6 g/d (Treatment 2) as a completely randomized design. Statistical significance was compared between the control and GABA treatment groups using the method of repeated measurement. Increased levels of rumen-protected GABA supplementation tended to mitigate the decline in milk yield associated with heat stress (p = 0.083). Milk fat content in the GABA supplementation groups was significantly greater than that in the control group (p = 0.036). Milk lactose content was significantly increased by GABA supplementation (p = 0.017). Blood metabolic profiles and cortisol did not differ significantly between the control and GABA supplementation groups. Activities in the GABA supplementation groups were significantly greater than those in the control group (p < 0.05). Rest and rumination times in the GABA supplementation group were significantly lower than those in the control group (p < 0.05). These results suggest that rumen-protected GABA can be a practical nutritional intervention for minimizing productivity losses in Holstein cows during periods of elevated ambient temperature.