# The Effect of Training on the Expression of Protein and Metabolites in the Plasma Exosomes of the Yili Horse

## TL;DR

This study shows that training Yili horses changes the proteins and metabolites in their plasma exosomes, which could help improve training and performance evaluation.

## Contribution

The study provides new insights into how training affects exosomal protein and metabolite profiles in Yili horses using multi-omics analysis.

## Key findings

- Training increased proteins related to muscle function, repair, metabolism, and immune regulation in Yili horse exosomes.
- Carbohydrates and their metabolites in exosomes significantly decreased after training.
- Training altered exosomal protein and metabolite composition, suggesting potential biomarkers for performance evaluation.

## Abstract

The effects of training on the expression of extracellular vesicle proteins and metabolites in Yili horse plasma were detected and analyzed through proteomics and metabolomics. The data shows that proteins that promote muscle function and repair, regulate metabolism and immune function in the plasma exosomes of Yili horses significantly increased after training, while metabolites related to carbohydrates and their metabolites significantly decreased. Combined analysis of proteome and metabolome data revealed that training had similar effects on the biological functions of proteins and metabolites in exosomes, indicating that training significantly altered the protein and metabolite composition of Yili horse exosomes.

Objective: The effects of physical exercise on protein expression and metabolites in the plasma exosomes of Yili horses are not yet clear. This study aims to elucidate the effects of exercise training on the expression of plasma extracellular vesicle proteins and metabolites in Yili horses through multi-omics analysis, providing reference indicators for the training and performance evaluation of Yili horses. Methods: Eight healthy 3-year-old Yili horses were selected for the study, with four untrained horses as the control group and four trained horses as the training group. After conducting proteomic and metabolomic analyses of the plasma exosomes of each group of horses, the differences in protein expression and metabolites were analyzed. Results: Compared with the control group, training significantly increased the levels of proteins in the plasma exosomes of Yili horses that promote muscle function and repair and regulate metabolism and immune function. In addition, carbohydrates and their metabolites were significantly reduced. Conclusion: Training significantly altered the protein composition and metabolites of Yili horse exosomes. These changes mainly provide new insights for the training and evaluation of Yili horse exercise ability, but further research is still needed.