# Transcriptome profiling across 6 different tissues in Vigna radiata var. sublobata

## TL;DR

This study provides a detailed transcriptome profile of six tissues in a wild mung bean variety, offering insights into gene expression patterns for potential crop improvement.

## Contribution

The study presents a comprehensive transcriptomic analysis of six tissues in Vigna radiata var. sublobata with biological replicates, revealing differentially expressed genes and tissue-specific gene functions.

## Key findings

- PCA showed distinct separation trends among the six tissue types.
- 19,405 differentially expressed genes were identified across tissues.
- Seed tissue showed up-regulated genes related to energy metabolism and storage compared to leaf tissue.

## Abstract

Mung bean (Vigna radiata) is a leguminous crop of significant economic, nutritional, and ecological importance. Its wild relative, V. radiata var. sublobata, is a wild legume exhibiting strong environmental adaptability and potential economic value. Its rich genetic diversity serves as a critical resource for research on crop stress resistance, thereby offering valuable wild genetic material for mung bean varietal improvement. In this study, we generated transcriptome sequencing data from six distinct tissues of V. radiata var. sublobata, providing a fundamental data for functional genomics research and molecular breeding applications.

In this study, we performed systematic transcriptomic sequencing analysis on six different tissues of V. radiata var. sublobata, with three biological replicates for each tissue type. Principal component analysis (PCA) demonstrated a significant separation trend among different tissue samples. A total of 19,405 differentially expressed genes (DEGs) were identified through pairwise cross-tissue comparisons. Gene Ontology (GO) enrichment analysis revealed that, relative to leaf tissue, seed tissue exhibited significant up-regulation of genes implicated in energy metabolism, storage processes, and developmental regulation. This study not only provides a tissue-specific gene expression profile for V. radiata var. sublobata but also offers important theoretical foundations and genetic resources for the utilization of wild legume plant resources and molecular breeding.