Phosphate-solubilizing Bacillus subtilis Y31 promotes cucumber growth and yield: insights from rhizosphere microbiomics and bacterial genomics

TL;DR

A new strain of Bacillus subtilis improves cucumber growth and yield by making phosphorus more available and altering soil fungi.

Contribution

A novel Bacillus subtilis strain, Y31, is shown to enhance cucumber growth through phosphorus solubilization and microbial community modulation.

Findings

Inoculation with Y31 increased greenhouse cucumber yield by 35.30%.

Y31 altered soil fungal communities, reducing harmful genera and increasing Penicillium.

Genome analysis revealed genes linked to phosphorus cycling and plant growth promotion.

Abstract

Phosphate-solubilizing bacteria (PSB) play a vital role in sustainable agriculture by enhancing plant growth and improving crop yield. In intensive cucumber (Cucumis sativus L.) cultivation systems, soil degradation commonly occurs, making the optimization of phosphorus availability a key strategy for increasing production. However, studies examining the growth-promoting effects of PSB in cucumber remain limited. In this study, we isolated a novel PSB strain, Y31, from cucumber rhizosphere soil and identified it as Bacillus subtilis. Strain Y31 demonstrated the ability to solubilize calcium phytate and calcium phosphate, secrete multiple enzymes, produce siderophores, and exhibit antagonistic activity against pathogenic fungi. Inoculation with Y31 significantly promoted cucumber growth, increasing greenhouse yield by 35.30%. Notably, Y31 application increased soil available phosphorus and altered the abundance of soil fungal community. It reduced the relative abundance of Botryotrichum and Chrysosporium, while promoting the relative abundance of the Penicillium genus. Genome sequencing of B. subtilis Y31 revealed the presence of genes involved in phosphorus cycling, carbohydrate-active enzymes, and the biosynthesis of 10 secondary metabolites. Together, these findings indicated that B. subtilis Y31 enhanced cucumber growth and yield by improving phosphorus availability, modulating microbial community structure, and carrying gene clusters linked to phosphorus solubilization and plant growth promotion. Therefore, this study provided a basis for the efficient utilization of phosphorus resources and supported the development of sustainable agricultural practices.

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