Correction: A motif unique to the human dead-box protein DDX3 is important for nucleic acid binding, ATP hydrolysis, RNA/DNA unwinding and HIV-1 replication

Abstract

Figures

A](#pone.0341385.g002), the first author AG stated that the incorrect image was erroneously included in [[1](#pone.0341385.ref001)] when preparing the originally published figure, and provided the original image panels intended to be included in [Fig 2A](#pone.0341385.g002) of [[1](#pone.0341385.ref001)]. They stated that the fractions used to conduct subsequent experiments (marked with an asterisk) were chosen based on the quantity and quality of protein purified from each fraction, and as such the replicate data for this figure provided in S1 File show different fractions with strongest sign

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After publication of this article [1], concerns were raised about Figs 2–4.

Specifically, when color levels and/or aspect ratio are altered:

In editorial follow-up, regarding Fig 2A, the first author AG stated that the incorrect image was erroneously included in [1] when preparing the originally published figure, and provided the original image panels intended to be included in Fig 2A of [1]. They stated that the fractions used to conduct subsequent experiments (marked with an asterisk) were chosen based on the quantity and quality of protein purified from each fraction, and as such the replicate data for this figure provided in S1 File show different fractions with strongest signal. They also stated that the vertical discontinuities in Figs 3A–3C were due to the removal of a blank lane between lanes 1 and 2, as supported by the underlying images; they indicated this may also apply to Fig 2D. Regarding the vertical discontinuity in Fig 3E, the first author stated this was likely due to removal of a repeated control lane.

The first author AG stated that Fig 4A was composed of images originating from three separate thin layer chromatography (TLC) plates. The explanations provided are supported by the available original and repeat image data underlying these figures. The original images underlying Figs 2D and 3E are not available; however, PLOS considers the explanation consistent with the information and data provided for the other figure panels.

Additionally, the first author AG notified the journal that lanes 2–5 in Fig 4F have been swapped with lanes 6–9 so that the lane order was consistent with Fig 4E. With this Correction, the first author AG has provided updated Figs 2–4 and associated figure captions, with splicing between lanes from the same scanned TLC plates clearly marked and lanes derived from separate TLC plates presented in separate panels, each with the control lane from the same plate.

The first author AG stated that the original image data are unavailable for Figs 2D, 3E, 4B–4D, 4G–4H, 6A–6B, and 7, and no quantitative data are available for Figs 2–5 and 7. They stated that original image data are available for Figs 2E, 2G, 3A–3C, 3F, 4A, 4E–4F, and 5A–5B, that both original and repeat data are available for Figs 2A–2B and repeat data only are available for Fig 2C and 3E, stated to be from experiments completed during the same period as the original experiments.

The authors apologize for the errors in the published article.

The corresponding author is deceased. Correspondence regarding this article may be sent to the first author, AG (anna.garbelli@cnr.it).

Supporting information

S1 FileUnderlying and replicate data.This file includes the original figure prepared for Fig 2, the original underlying image data for Figs 2A–B (photographs of laboratory notebook), 2E, 2G, 3A–C, 3F, 4A, 4E–F and 5A–B. Repeat data underlying Figs 2A–C, and 3E.(PDF)