# Cardiac puncture blood collection as a practical and biosecure method for post-mortem pathogen detection in pigs

## TL;DR

This paper introduces cardiac puncture as a practical and biosecure method for collecting blood from dead pigs to detect pathogens during outbreaks.

## Contribution

The study demonstrates the feasibility of using cardiac puncture for post-mortem pathogen detection in pigs without environmental contamination.

## Key findings

- Blood was successfully collected from 286 dead pigs using cardiac puncture.
- 95% of the tested samples from the collected blood showed positive virus detection.
- The method avoids blood spillage and environmental contamination during pathogen investigations.

## Abstract

Live pig specimen collection can be time-consuming as it may require physical restraining. Non-invasive methods for live animals that don't involve blood spillage are available but some may present limitations of application or pathogen detection. Alternative methods focused on the mortality are also available but may derive in blood spillage. As biocontainment and bioexclusion are of concern, especially for pathogens such as the ones that cause classical swine fever and African swine fever, alternative methods can be applied. This article presents the results and opinions of the use cardiac puncture (CP) as an alternative of specimen collection from dead pigs. This method was used in two separate studies involving porcine reproductive and respiratory virus detection from blood of dead pigs of different ages. Blood was successfully obtained from 286 suckling and growing pigs, that were euthanized or died during outbreaks of the tested virus. While only 273 samples could be tested due to the other 13 not yielding enough sera of having inhibitors, of those 95% had positive detections for the virus. This method was not only feasible for obtaining blood and test the sera, but also avoided environmental contamination with blood, offering an alternative tool for collection during outbreak investigations.