# Proteomic and metabolomic profiling reveals the fungicidal mechanisms of Microsporum canis in response to methylene blue-mediated photodynamic therapy

## TL;DR

This study uses proteomic and metabolomic profiling to uncover how methylene blue photodynamic therapy kills Microsporum canis fungi by disrupting multiple cellular processes.

## Contribution

The paper provides the first system-level understanding of MB-PDT's fungicidal mechanism in M. canis through integrated multi-omics analysis.

## Key findings

- MB-PDT disrupts membrane integrity by downregulating ergosterol biosynthesis enzymes like C4-methylsterol oxidase.
- The treatment suppresses antioxidant defenses and virulence factors such as subtilisin-like protease 7.
- MB-PDT inhibits ribosome biogenesis and translation, leading to a collapse in protein synthesis and amino acid metabolism.

## Abstract

Microsporum canis is a primary causative agent of dermatophytosis. Its rising antifungal resistance necessitates the development of effective therapeutic alternatives. Although methylene blue-mediated photodynamic therapy (MB-PDT) is a promising strategy, a system-level understanding of its fungicidal mechanism is lacking.

An integrated multi-omics approach was employed, using data-independent acquisition (DIA) proteomics and untargeted metabolomics, to map the molecular response of clinical M. canis isolates to MB-PDT. Pathway enrichment analysis was performed to elucidate the key biological processes affected.

MB-PDT induced multi-faceted molecular perturbations in M. canis. The treatment simultaneously disrupted membrane integrity by downregulating ergosterol biosynthesis (e.g., C4-methylsterol oxidase) and impaired the fungus’s antioxidant defenses by suppressing key enzymes such as glutathione S-transferase. Critically, the treatment suppressed secreted virulence factors essential for host invasion, including subtilisin-like protease 7. These disruptions led to a profound suppression of core biosynthetic machinery, with ribosome biogenesis and translation identified as the most significantly inhibited pathways. This resulted in a collapse of protein synthesis, energy production, and amino acid metabolism.

The results indicate that the efficacy of MB-PDT stems from a multi-target mechanism that simultaneously damages cellular structures, attenuates virulence, and dismantles the fungus’s metabolic and translational capacity. This contrasts sharply with single-target conventional antifungals, providing a strong molecular rationale for its low potential to induce resistance. This study offers a comprehensive molecular blueprint for the action of MB-PDT against M. canis, strongly supporting its development as a durable therapeutic strategy for dermatophytosis.