# The role of total polysaccharides from Sonchus arvensis L. in the prevention and treatment of colitis via regulation of gut microbiota and metabolites

## TL;DR

This study shows that polysaccharides from Sonchus arvensis L. may help treat colitis by improving gut microbiota and metabolites.

## Contribution

The novel finding is that SAP treatment reverses gut microbiota changes and regulates specific metabolites in a colitis model.

## Key findings

- SAP treatment attenuated colitis symptoms and improved immune parameters in mice.
- SAP reversed the abundance of specific gut microbiota like Akkermansia and Rikenellaceae_RC9_gut_group.
- SAP regulated five metabolites that are correlated with gut microbiota changes.

## Abstract

Sonchus arvensis L. is a traditional Chinese food and medicine, and the primary plant metabolites are polysaccharides (SAP). In this study, we aimed to investigate the protective effect of SAP in a colitis model and the potential underlying molecular mechanisms.

C57BL/6 mice were randomly assigned to three groups: the negative control, model, and SAP treatment groups. The influence of SAP on ulcerative colitis (UC) was evaluated by water and food intake, body weight change, diarrhea, bloody stool, colon length, histological analysis, disease activity index, and immune parameters. The effects of SAP on the gut microbiota (GM) were investigated using 16S rRNA sequencing. The impact of SAP on metabolites was evaluated using non-targeted metabolomics analysis.

SAP attenuated colitis and modified immune parameters. 16S rRNA sequencing showed that the abundance of Akkermansia, Rikenella, Rikenellaceae_RC9_gut_group, and unidentified_Clostridia_vadinBB60_group in the model mice was remarkably reversed after SAP treatment. The correlation analysis of GM and the metabolites showed that SAP could regulate five metabolites correlated with GM.

The protective effect of SAP on the model mice may be related to GM diversity and metabolites.