# Anti-Acne Potential of Quercus salicina Extract: Inhibition of Cutibacterium acnes Growth and Virulence

## TL;DR

This study shows that an extract from Quercus salicina can inhibit the growth and harmful effects of Cutibacterium acnes, a bacteria linked to acne.

## Contribution

The study identifies active compounds in Quercus salicina extract that inhibit C. acnes virulence factors.

## Key findings

- QsB-EE showed strong antimicrobial activity with a MIC of 125 μg/ml.
- The E1 fraction significantly downregulated key virulence genes in C. acnes.
- LC-MS/MS identified (+)-catechin as the major compound in the most effective E1 fraction.

## Abstract

Acne vulgaris is a prevalent inflammatory skin disorder mediated by Cutibacterium acnes, a key etiological factor. In this study, the anti-acne properties of the ethyl acetate fraction of Quercus salicina Blume (QsB-EE) against C. acnes were investigated. The QsB-EE fraction was separated into subfractions (E1-E6) using preparative HPLC. Antimicrobial and anti-virulence activities were evaluated via bacterial growth, biofilm, and lipase activity assays. Virulence gene expression was assessed using qRT-PCR, and bioactive compounds were identified by LC-MS/MS. The QsB-EE demonstrated significant antimicrobial (MIC 125 μg/ml) and strong anti-virulence effects. The E1 fraction was the most potent, exhibiting the lowest MIC (16 μg/ml), highest biofilm inhibition (86.19% at 1,000 μg/ml), and highest lipase inhibition (93.72% at 10 μg/ml). In addition, mechanistic studies confirmed that E1 significantly downregulated the expression of key virulence genes: ppa0149 (biofilm), gehA (lipase), and hyl (hyaluronidase). LC-MS/MS identified (+)-catechin as the major compound in E1, alongside kaempferol and quercetin in E5. Although further in vivo studies are warranted to evaluate the therapeutic efficacy, our findings suggest that QsB-EE possesses promising anti-acne potential by targeting C. acnes virulence factors through the active compounds concentrated in the E1 fraction.