# Stimulation of Human Adenovirus Infection Modulated by Emerging Micropollutants

## TL;DR

This study shows that certain environmental pollutants can boost the replication of a human adenovirus under specific temperature conditions.

## Contribution

The study reveals temperature-dependent enhancement of adenovirus replication by micropollutants in wastewater-like conditions.

## Key findings

- Co-incubation of CECs with HAdV-5 at 37°C increased viral replication by up to 1.5 log₁₀.
- No significant viral replication enhancement was observed at room temperature.
- CECs modulate viral infectivity through direct physicochemical interactions in aquatic environments.

## Abstract

Contaminants of emergent concern (CECs), such as pharmaceuticals and microplastics, are increasingly found in aquatic environments, yet their interactions with viral pathogens remain underexplored. This study evaluated the effects of antibiotics, antidepressants, microfibers, and nanoplastics on human adenovirus type 5 (HAdV-5) replication in A549 cells. A series of in vitro assays simulating distinct exposure scenarios across the viral replication cycle was conducted. Results showed that individual pre- or post-infection exposure to CECs did not significantly impact HAdV-5 replication. However, co-incubation of CECs with viral particles at physiological temperature (37 °C) led to a significant increase in viral replication up to 1.5 log₁₀ compared to viral control, highlighting temperature-dependent interactions. No enhancement was observed at room temperature. The findings suggest that CECs can modulate viral infectivity through direct physicochemical interactions, particularly under conditions resembling those of wastewater environments. This study provides new insights into the potential risks posed by the co-occurrence of viruses and CECs in aquatic ecosystems.