Legacies of consecutive summer droughts on soil‐borne plant parasitic protists (Oomycota: Stramenopila and Phytomyxea: Rhizaria) and protistan consumers (Cercozoa: Rhizaria) along an experimental plant diversity gradient

TL;DR

Repeated summer droughts and plant diversity loss affect soil-borne plant parasites and consumers, potentially harming grassland ecosystems.

Contribution

The study reveals long-lasting soil legacy effects of drought on protistan parasites and consumers in diverse plant communities.

Findings

Oomycota and Cercozoa responded to plant diversity and drought, with species-specific Oomycota enriched under drought.

Phytomyxea and cercozoan consumers showed shifts in operational taxonomic units under drought.

Plant diversity did not mitigate the negative effects of drought on protistan communities.

Abstract

Increasing frequencies of severe summer droughts and plant diversity loss disrupt ecosystem functioning and stability of European grasslands. Understanding how these factors interact with pathogens is crucial.We investigated the effects of plant diversity and repeated summer drought on soil‐borne parasites within a grassland biodiversity experiment. The experiment included plant communities ranging from 1 to 60 species, with a sub‐experiment simulating annual droughts for 6 wk in summer over 9 yr. One year after the final drought period, we analyzed the diversity and community composition of two parasitic protistan taxa with many plant‐pathogenic members, Oomycota (Stramenopila) and Phytomyxea (Rhizaria), as well as protistan consumers in the Cercozoa (Rhizaria) using amplicon sequencing.Both Oomycota and Cercozoa, including Phytomyxea, responded to plant species richness and drought, but not uniformly. Plant species‐specific Oomycota were enriched under drought, while Phytomyxea and cercozoan consumers exhibited shifts in both enriched and reduced operational taxonomic units. No mitigating effect of plant diversity against the effects of drought was observed.Our findings suggest that repeated summer droughts weaken plant defense against protistan plant parasites, causing long‐lasting soil legacy effects across plant communities varying in diversity and community composition, potentially threatening ecosystem stability and functioning under future climate conditions.

Increasing frequencies of severe summer droughts and plant diversity loss disrupt ecosystem functioning and stability of European grasslands. Understanding how these factors interact with pathogens is crucial.

We investigated the effects of plant diversity and repeated summer drought on soil‐borne parasites within a grassland biodiversity experiment. The experiment included plant communities ranging from 1 to 60 species, with a sub‐experiment simulating annual droughts for 6 wk in summer over 9 yr. One year after the final drought period, we analyzed the diversity and community composition of two parasitic protistan taxa with many plant‐pathogenic members, Oomycota (Stramenopila) and Phytomyxea (Rhizaria), as well as protistan consumers in the Cercozoa (Rhizaria) using amplicon sequencing.

Both Oomycota and Cercozoa, including Phytomyxea, responded to plant species richness and drought, but not uniformly. Plant species‐specific Oomycota were enriched under drought, while Phytomyxea and cercozoan consumers exhibited shifts in both enriched and reduced operational taxonomic units. No mitigating effect of plant diversity against the effects of drought was observed.

Our findings suggest that repeated summer droughts weaken plant defense against protistan plant parasites, causing long‐lasting soil legacy effects across plant communities varying in diversity and community composition, potentially threatening ecosystem stability and functioning under future climate conditions.

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