Scalable Multiparametric Characterization of Aptamer–Target Interactions
Marc Sulliger, Matthew Peters, Andrea Sottini, Annina Stuber, Kyungae Yang, Nako Nakatsuka, Jaime Ortega Arroyo, Romain Quidant
TL;DR
This paper introduces a scalable platform to study how aptamers change shape when binding targets, enabling better design of biosensors for detecting small molecules.
Contribution
A scalable microfluidic platform integrating FRET and imaging for high-resolution profiling of aptamer–target interactions.
Findings
The platform enables detailed analysis of aptamer–target interactions in picoliter volumes across millisecond-to-hour timescales.
Structure–function relationships of serotonin aptamers were systematically explored to select optimal candidates for biosensing.
The system bridges low-throughput structural analysis with rapid, multiparametric readouts for translational biosensor development.
Abstract
Structure-switching aptamers transduce target-induced conformational changes into detectable signals, enabling the specific detection of small molecules with limited surface area and charge. Understanding these structural transitions is critical for the rational design of aptamers in downstream biosensing. However, current methods lack the scalability and high spatiotemporal resolution to characterize and resolve these structural dynamics within a single unified platform. Here, we report a scalable droplet microfluidic platform that fills this technological gap by integrating Förster resonance energy transfer with automated imaging for the multiparametric profiling of aptamer–target interactions. This integrated system enables the detailed analysis of aptamer–target interactions in picoliter volumes under physiologically relevant conditions across the millisecond-to-hour time scales.…
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Taxonomy
TopicsAdvanced biosensing and bioanalysis techniques · Innovative Microfluidic and Catalytic Techniques Innovation · Advanced Biosensing Techniques and Applications