Gut Microbiome Diagnostic Biomarkers for Colorectal Cancer

TL;DR

This study identifies gut bacteria like Fusobacterium nucleatum and Peptostreptococcus anaerobius as potential noninvasive biomarkers for diagnosing colorectal cancer and tracking its progression.

Contribution

The study introduces a novel noninvasive CRC diagnostic approach using gut microbiome biomarkers validated across healthy, adenoma, and CRC patient groups.

Findings

Fusobacterium nucleatum and Peptostreptococcus anaerobius show strong correlations with CRC stages and metastasis.

P. anaerobius combined with Fn provides the highest diagnostic accuracy for CRC detection.

A treatment efficacy and prognostication model was developed using statistical methods.

Abstract

Gold standard diagnostic methods, such as invasive procedures and serum biomarkers, have limited sensitivity and specificity for the detection of colorectal cancer (CRC). Thus, the development of more accurate and noninvasive detection approaches is imperative. Emerging research elucidating the intricate role of the gut microbiota in CRC pathogenesis underscores the need for precision screening tailored to high-risk cohorts to improve early detection and intervention strategies and comprehensively address this challenging clinical problem.

Fecal metagenomic sequencing datasets were employed to identify potential bacterial biomarkers for CRC diagnosis and selected relevant microbial taxa for subsequent validation. A total of 180 participants were enrolled: 65 healthy controls (HC), 65 colorectal adenoma patients, and 50 CRC patients, and fecal samples were analyzed using fluorescence quantitative polymerase chain reaction to confirm biomarker relative abundance, culminating in the establishment of an evolutionary model for CRC progression; furthermore, a treatment efficacy and prognostication model supported by comprehensive statistical methodologies was established.

This study analyzed fecal microbial biomarkers associated with CRC progression and identified differentially abundant bacterial species across HCs, adenoma, and CRC patient groups. Notably, Fusobacterium nucleatum (Fn) and Peptostreptococcus anaerobius (P. anaerobius) showed significant correlations with CRC stage and metastasis, highlighting their potential as diagnostic biomarkers. Among individual microbes, P. anaerobius exhibited the highest diagnostic value when combined with Fn.

The results underscore the potential application of fecal microbial markers, particularly Fn and P. anaerobius, for diagnosing CRC and monitoring its progression.

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