Biocompatibility of Nickel Ferrite Nanoparticles on Systemic and Testicular Cells
Carla Cristina Martins Silva, Pedro Igor Macário Viana, Thalita Marcolan Valverde, José Domingos Ardisson, Daniele Alves Fagundes, Guilherme Mattos Jardim Costa
TL;DR
This study examines how nickel ferrite nanoparticles affect the viability and function of systemic and testicular cells, revealing potential risks and mechanisms of toxicity.
Contribution
The study provides new insights into the biocompatibility and internalization mechanisms of nickel ferrite nanoparticles in male reproductive cells.
Findings
FeNi NPs at 500 μg/mL reduced the viability of systemic and male reproductive cells.
At 100 μg/mL, FeNi NPs increased cell death via apoptosis and necrosis in Leydig and germ cells.
FeNi NPs were rapidly internalized in testicular cells, observed via TEM, and increased reactive oxygen species.
Abstract
Nickel ferrite nanoparticles (NPs) are chemically stable and have a surface suitable for functionalization, making them potential biotechnological tools in male reproduction. Herein, the nickel ferrite (FeNi) NPs were synthesized by the hydrothermal method and presented colloidal stability, low aggregation, high crystallinity, and superparamagnetic behavior at room temperature. At a dose of 500 μg/mL, FeNi NPs reduced the viability of systemic and male reproductive cells at all time points. We selected 100 μg/mL to further investigate its effects on testicular cells, as it was safe for VERO and AML-12 cells. For male reproductive cells, we observed that the selected FeNi NP dose significantly increased cell death by apoptosis and necrosis in Leydig cells, and by apoptosis in germ cells. We observed rapid internalization of FeNi NPs in both cell types within the first 5 min of exposure.…
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Taxonomy
TopicsNanoparticle-Based Drug Delivery · Magnetic Properties and Synthesis of Ferrites · Iron Metabolism and Disorders