Integrated metabolomic and transcriptomic profiling elucidates the tissue-specific biosynthesis and regulation of flavonoids in Machilus nanmu
Xiao Zhang, Changying Xia, Huan Zhang, Wenqiao Li, Zhe Zhang, Nana Long, Renxiu Yao, Jian Li, Hongping Deng
TL;DR
This study combines metabolomic and transcriptomic data to understand how flavonoids are made in different tissues of Machilus nanmu, a tree with industrial and health potential.
Contribution
The study provides the first comprehensive model of tissue-specific flavonoid biosynthesis and identifies key genes and transcription factors in Machilus nanmu.
Findings
425 flavonoid compounds and 35,671 differentially expressed genes were detected across tissues.
Two UGTs were localized to the cytoplasm, and four transcription factors were identified as key regulators of flavonoid biosynthesis.
WGCNA revealed four gene modules strongly correlated with flavonoid content.
Abstract
Machilus nanmu is a significant arborescent species of the genus Machilus (Lauraceae), exhibiting considerable potential for applications in industrial materials and healthcare. However, systematic investigations into its flavonoid metabolites and associated biosynthetic mechanisms remain limited, which significantly hinders the efficient exploitation and sustainable utilization of this species. This multi-omics study revealed the specific accumulation pattern of flavonoids in the tissues of M. nanmu and pinpointed key structural and regulatory genes underlying their biosynthesis by integrating widely targeted metabolomics and transcriptomics data from roots, stems, and leaves. A total of 425 flavonoid compounds and 35,671 differentially expressed genes were detected. Further screening revealed 41 structural genes encoding 19 key enzymes (including PAL, CHS, FLS, UGTs, etc.), among…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsPlant Gene Expression Analysis · Traditional and Medicinal Uses of Annonaceae · Plant-derived Lignans Synthesis and Bioactivity