# Fed the same way? Exploring the influence of breastfeeding, weaning, and childhood diet on adult sex ratios through stable isotope analysis of incremental dentine in Medieval Tuscany, Italy (11th–15th c. CE)

**Authors:** Alessio Amaro, Antonio Fornaciari, Valentina Giuffra, Sang-Tae Kim, Martin Knyf, Paul Szpak, Bonnie Kahlon, Tracy L. Prowse, Dario Piombino-Mascali, Dario Piombino-Mascali, Dario Piombino-Mascali

PMC · DOI: 10.1371/journal.pone.0338595 · PLOS One · 2026-01-21

## TL;DR

This study uses isotope analysis of medieval teeth to show that boys were weaned earlier than girls, but both had similar childhood diets, with no major disadvantage for girls.

## Contribution

The study provides new insights into medieval gender-specific weaning practices and their potential impact on adult sex ratios.

## Key findings

- Males were weaned earlier than females across all three medieval sites in Tuscany.
- Both genders had similar early diets, with greater variability after weaning.
- Breastfeeding and weaning patterns changed over time in the medieval period.

## Abstract

In this paper we investigate whether infant and childhood feeding practices influenced the imbalanced adult sex ratio reported in medieval Europe from historical and osteological evidence. First, we examine hypotheses for the observed imbalanced sex ratios in Europe and the evidence presented to support these hypotheses. We then use stable isotope analysis (δ13C and δ15N) of incremental dentine in 64 first molars from adults at three medieval sites (Aulla, Badia Pozzeveri, and Montescudaio) in north-western Tuscany (11th-15th c. CE) to investigate the timing and pattern of breastfeeding and weaning. Our results show that males were exclusively breastfed for a shorter period and were weaned earlier than females in all three samples (Aulla M: 2.2 yrs, F: 2.6 yrs; Badia Pozzeveri M: 2.3 yrs, F: 3.0 yrs; Montescudaio M: 2.2 yrs, F: 2.9 yrs). In addition, both males and females consumed an isotopically similar diet early in life with greater variability during the post-weaning years. We explore how gender differences in the social roles of young males and females may have influenced infant and childhood feeding practices. It appears that these practices did not significantly disadvantage young females. In addition, we do see evidence for changing patterns of breastfeeding and weaning over time.

## Full-text entities

- **Chemicals:** delta13C (-)

## Full text

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## Figures

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## References

118 references — full list in the complete paper: https://tomesphere.com/paper/PMC12822958/full.md

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Source: https://tomesphere.com/paper/PMC12822958