# Characterization of Polysaccharides Derived From Cinnamomum cassia and Their Potential of Antioxidant Activity and Protective Action Against Methomyl Induced Rats Damage With In Silico Docking

## TL;DR

This study explores the antioxidant and protective properties of polysaccharides from Cinnamomum cassia against methomyl-induced damage in rats.

## Contribution

The study introduces a novel in silico docking approach to evaluate the bio-insecticidal potential of Cinnamomum cassia polysaccharides.

## Key findings

- Cc-Ps showed high scavenging activity against DPPH●, ABTS•+, and NO free radicals.
- Pretreatment with Cc-Ps normalized oxidative stress markers and histopathological damage caused by methomyl.
- In silico docking simulations confirmed the bio-insecticidal potential of Cc-Ps compounds.

## Abstract

Herbal medicine is an important field focused on the treatment and prevention of various health conditions that may occur due to oxidative stress. Our investigation aimed to determine the chemical composition, antioxidant, and the protective activity of polysaccharides isolated from Cinnamomum cassia (Cc‐Ps). Cc‐Ps underwent thorough characterization through various analytical methods, including UV, Fourier Transform Infrared (FTIR) spectroscopy, and HPLC. Our analysis showed that the Cc‐Ps exhibited highest amount of uronic acid. In addition, Cc‐Ps extract showed high ability to scavenge 2,2 diphenylpicrylhydrazyl radical (DPPH●), 2,2′‐azinobis‐(3 ethylbenzothiazoline‐6 sulfonic acid radical (ABTS•+), and NO free radicals in reaction system. There was a noticeable decrease in the activity of key antioxidant enzymes such a superoxide dismutase and glutathione (GSH) peroxidase accompanied by an increase in malondialdehyde, a marker of oxidative stress. MET led to a clear impairment of the histopathological structure of both the liver and kidneys. Pretreatment with the polysaccharide extract normalized all biochemical and oxidative parameters. Virtual screening using docking simulations against “Drosophila melanogaster Acetylcholinesterase” was conducted to assess the bio‐insecticidal properties of specific polysaccharide compounds derived from C. cassia. Polysaccharides were effectively protective in the liver and kidneys against the toxic effects of MET under our experimental conditions.

Cinnamomum cassia and Their Potential of Antioxidant Activity and Protective Action Against Methomyl Induced Rats Damage With In Silico Docking