# Anuran genome size evolution is driven by relatively recent retrotransposon activity and by life history

## TL;DR

This study shows that frog and toad genome size differences are mainly due to recent retrotransposon activity and developmental traits like larval period.

## Contribution

The study identifies specific retrotransposon families and life history traits as key drivers of genome size evolution in anurans.

## Key findings

- Copy numbers of specific transposable element families are linked to increased genome size in frogs and toads.
- Recently active Ty3 retrotransposons correlate with genome size expansion in anurans.
- Interactions between transposable elements and larval period suggest developmental constraints influence genome size.

## Abstract

Transposable elements (TEs), often referred to as ‘selfish genetic elements’, hijack their host’s genetic machinery to replicate themselves within the host genome, and are primary contributors to genome size in vertebrates. In particular, frogs and toads (Order: Anura) are well known for not only having large genomes, but for having genomes with drastic variation in size (e.g., Scaphiopus couchii: 0.48 Gbp, Rana muscosa 10 Gbp). This discrepancy in size is due in part to relative differences in the proliferation and success of TEs across anuran genomes. In this study, we ask: do specific TE families drive genome size variation and are these patterns phylogenetically-constrained? To answer this, we use 61 publicly available anuran reference genome assemblies, comprising 22 anuran families, to investigate the effects of TEs on the evolution of anuran genome size. In addition, we used repeat landscapes to analyze TE activity in the context of anuran evolutionary history, in order to understand how lineage-specific TE activity affects genome size variation in anurans. Finally, we examined how life history traits that have been predicted to be constrained by genome size, larval period and clutch size, are associated with TEs.

Our results suggest that copy number of elements and total content of several TEs families (L1/CIN4, Ty1/Copia, Ty3/DIRS1, hobo-Activator) are associated with increased genome size across anurans. We further found that the copy numbers of recently active Ty3 retrotransposons are correlated with increased genome size, suggesting that expansions of this family of TE have had a distinct effect on the size of the anuran genome across evolutionary time. Finally, we found interactions between several TE families (hobo-Activator, PiggyBac, L2/CR1/Rex, and SINEs) and larval period, indicating that developmental time may be a major constraint on genome size and species with additional constraints may more effectively purge TEs.

Our findings underscore the pivotal roles of both the activity of specific TE families (L1/CIN4, Ty1/Copia, Ty3/DIRS1, hobo-Activator) and developmental constraints in amphibian genome size evolution.

The online version contains supplementary material available at 10.1186/s12864-025-12414-y.