# Decoding Cerebrospinal Fluid: Integrative Metabolomics Across Multiple Platforms

## TL;DR

This paper introduces a standardized method to study cerebrospinal fluid using multiple technologies to better understand brain chemistry.

## Contribution

A new integrative multiplatform metabolomic workflow combining 1H-NMRS and HPLC-MS for CSF analysis is introduced.

## Key findings

- Integrating 1H-NMRS and HPLC-MS improves metabolite coverage in CSF.
- The workflow enables reliable characterization of the CSF metabolome.
- The method supports discovery of potential CNS biomarkers.

## Abstract

Cerebrospinal fluid (CSF) is a key biological matrix that reflects the physiological and pathological states of the central nervous system (CNS). It supports brain function by regulating ionic balance, facilitating molecular transport, and clearing metabolic waste. In this article, we present a standardized protocol for CSF collection along with an integrative multiplatform metabolomic workflow that combines proton nuclear magnetic resonance spectroscopy (1H-NMRS) and high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Integrating these complementary analytical modalities enhances metabolite coverage and improves analytical robustness, enabling a more comprehensive and reliable characterization of the CSF metabolome. This workflow supports the discovery of potential biomarkers and advances our understanding of neurochemical alterations within the CNS.