# Characterization of occupational exposure to airborne particles and bioaerosols in dental clinics

## TL;DR

This study examines airborne particles and bioaerosols in dental clinics to assess health risks for dental professionals.

## Contribution

The study provides new insights into particle size, concentration, and composition during dental procedures in real clinical settings.

## Key findings

- Respirable particle mass concentrations were below Norwegian occupational exposure limits but ultrafine particles increased during procedures.
- Bacterial and fungal DNA concentrations varied significantly across dental clinics.
- Air polishing activities were linked to peak particle concentrations in one clinic.

## Abstract

Occupational exposure to airborne particles and bioaerosols in dental clinics is a potential hazard to dental health workers. Current studies on airborne particles and bioaerosols in dental clinics are limited and methodologically diverse, leaving gaps in the understanding of airborne particles in real-life dental settings. The aim of the study was to investigate the size, concentration, and composition of particles produced during dental procedures, and determine the exposure levels of dental personnel to respirable particles and bioaerosols in dental clinical environments with different characteristics. The study included two conventional dentist offices and one specialty clinic. The number concentration and size distribution of particles released during different dental procedures were monitored in real-time in dental procedure rooms. Personal samplers were used in parallel to collect the respirable and inhalable particle fractions. Total bacterial and total fungal DNA concentrations were quantified in the inhalable particle fraction by droplet digital polymerase chain reaction. Particle morphology and chemical composition were analyzed using scanning electron microscopy. The highest geometric mean value of the respirable particle mass concentration (0.06 mg/m3) was below the Norwegian occupational exposure limit for respirable dust of 5 mg/m3. Real-time sampling indicated that particle number concentrations were elevated during working hours in two clinics, with peak levels observed in one clinic coinciding with air polishing activities. The results also showed significant variations in bacterial and fungal DNA concentration levels (P < 0.0001). Many collected particles originated from powders used in dental treatments. Despite low respirable particle mass concentrations, increased levels of ultrafine particles during dental procedures highlight potential health risks to dental professionals. These findings also underscore the importance of advanced ventilation and safety measures to mitigate occupational exposure in dental environments.