# Isolation, characterization, and annotation of two bacteriophage from North Carolina soil using Arthrobacter globiformis : HamCheese and Kihatsu

## TL;DR

Two new bacteriophages, HamCheese and Kihatsu, were isolated from North Carolina soil and characterized based on their genetic features.

## Contribution

The paper introduces and characterizes two novel siphoviruses, HamCheese and Kihatsu, isolated from NC soil using Arthrobacter globiformis.

## Key findings

- HamCheese has a 38,454 base pair genome encoding 67 putative genes and belongs to cluster AS.
- Kihatsu has a 43,237 base pair genome encoding 67 putative genes and 2 tRNAs, belonging to cluster FF.
- Both phages are predicted to be temperate based on gene content analysis.

## Abstract

Bacteriophage HamCheese and Kihatsu are siphoviruses isolated from soil in Raleigh, NC and Clayton, NC, respectively, using
Arthrobacter globiformis
, B2979. HamCheese is assigned to actinobacteriophage cluster AS based on gene content similarity. Its 38454 base pair genome encodes 67 putative genes. Kihatsu is assigned to cluster FF phage, with a genome 43,237 base pairs in length encoding 67 putative genes and 2 tRNAs. Based on gene content, both pages are predicted to be temperate.