# Isolation and genomic analysis of secondary metabolism in cave Actinomycetota from biofilms and Ceuthophilus

## TL;DR

This paper describes the isolation and genomic study of Actinomycetota from cave biofilms and crickets, revealing new bacterial diversity and secondary metabolism.

## Contribution

The study provides novel genomic insights into cave Actinomycetota and their secondary metabolism potential.

## Key findings

- Nine Actinomycetota strains were isolated from cave biofilms and crickets.
- Genomic analysis revealed diverse genera with low DNA-DNA hybridization to known species.
- The study highlights the secondary metabolism potential of cave Actinomycetota.

## Abstract

We report the isolation and genomic sequencing of nine Actinomycetota obtained from both cave biofilms and trogloxenic
Ceuthophilus
(cave crickets). Strains were isolated from samples via actinomycetota-selective media and sequenced using nanopore sequencing. Provisional taxonomic inference of strains was performed by whole genome phylogenetic analysis, revealing a broad range of genera, with most strains clustering with known genera with digital DNA-DNA hybridization values less than 70%, and one strain not clustering with known genera. An analysis of secondary metabolism reveals the quantity and diversity of secondary metabolism in cave Actinomycetota.