# Supplementation with commercially available probiotic bacterial strains reduces fat accumulation in Caenorhabditis elegans by regulating the lipid metabolism pathway

## TL;DR

This study shows that certain probiotic bacteria reduce fat accumulation in a type of worm by influencing lipid metabolism.

## Contribution

The novel finding is that EFT110, BSTOA, and CBTOA reduce fat accumulation in C. elegans by regulating lipid metabolism genes.

## Key findings

- EFT110, BSTOA, and CBTOA significantly reduce fat accumulation in C. elegans.
- These probiotics regulate gene expression in lipid metabolic pathways.

## Abstract

Enterococcus faecium

T-110 (EFT110),

Bacillus subtilis

TO-A (BSTOA), and

Clostridium butyricum

TO-A (CBTOA) are probiotic bacteria used as supplements to improve intestinal symptoms in humans, chickens, pigs, cattle, and fish. However, their effects on hosts remain unclear. Our previous study showed that EFT110, BSTOA, and CBTOA extend the lifespan of

Caenorhabditis elegans

. In this study, we investigated the effects of these three strains on fat accumulation in nematodes; and observed that each bacterium significantly reduced fat accumulation by regulating gene expression in lipid metabolic pathways.