# Cryo‐EM reveals an ensemble of cytochrome P450 reductase conformations in solution

## TL;DR

Cryo-EM reveals different shapes of a key enzyme involved in drug metabolism and biosynthesis.

## Contribution

First cryo-EM structure of rat CPR at 3.3 Å resolution, revealing conformational flexibility.

## Key findings

- Rat CPR adopts a compact conformation in solution, more relaxed than in crystal structures.
- About 20% of CPR molecules show the FMN-binding domain detached from the catalytic core.
- Cryo-EM reveals conformational variations that may explain CPR's electron transfer mechanism.

## Abstract

The eukaryotic electron transport system, mediated by cytochrome P450 reductase (CPR), plays a crucial role in driving myriads of reactions involved in the biosynthesis of physiologically active compounds (such as sterols, steroids, vitamins, and natural products), as well as in the metabolism of drugs, toxins, and carcinogens. CPR is a diflavin‐containing enzyme found ubiquitously on the cytosolic side of the endoplasmic reticulum. While several crystal structures of CPR are available, its conformational states in solution, along with the molecular details of action, remain debatable. Here, we determined the 3.3 Å cryo‐EM structure of rat CPR, marking the first electron microscopy structure of this relatively small protein (77 kDa). In this structure, the full‐length, fully active enzyme adopts a compact conformation, which, however, is more relaxed than in crystal structures. Moreover, we structurally characterized less populated variations of compact CPR conformations and identified a fraction of molecules (~20%) with the FMN‐binding domain either not visible or positioned far from the rest of the catalytic core. These results support the idea that large‐scale interdomain rearrangements serve as the structural basis for CPR function and suggest that cryo‐EM techniques can help uncover the intricate molecular mechanisms governing the CPR‐mediated electron transfer cycle.