# Deep learning-based no-reference image quality assessment framework for Cryptosporidium spp. and Giardia spp

**Authors:** Muhammad Amirul Aiman Asri, Heshalini Rajagopal, Norrima Mokhtar, Wan Amirul Wan Mohd Mahiyiddin, Yvonne Ai Lian Lim, Masahiro Iwahashi, Ryosuke Harakawa, Fatimah Ibrahim, Takao Ito, Ayush Dogra, Ayush Dogra, Ayush Dogra, Ayush Dogra, Ayush Dogra

PMC · DOI: 10.1371/journal.pone.0341160 · PLOS One · 2026-01-20

## TL;DR

This paper introduces a deep learning model to assess the quality of microscope images of Cryptosporidium and Giardia parasites, improving diagnostic accuracy in public health.

## Contribution

A novel NR-IQA model, PRIQA, specifically trained on a small parasite image dataset, outperforms existing methods in image quality assessment.

## Key findings

- ResNet-101 was identified as the most robust feature extractor for parasite image quality assessment.
- PRIQA outperformed ten state-of-the-art NR-IQA algorithms in evaluating parasite microscopy images.
- The model can serve as a practical quality control tool for automated parasite detection systems.

## Abstract

Image Quality Assessment (IQA) plays a critical role in image-based decision-making systems, especially in domains requiring high diagnostic precision. Effective feature information is a prerequisite for the high performance of machine learning methods in parasitic organism detection, and the quality of this feature information is influenced by the quality of the images. However, No-Reference IQA (NR-IQA) models have ignored microscopy-based datasets, particularly those involving parasitic organisms such as Cryptosporidium spp. and Giardia spp., which are vital for public health inspection. In this study, PRIQA (Parasite ResNet-101 IQA), a novel deep learning-based NR-IQA model specifically trained on a small parasite image dataset was presented. Using Mean Opinion Scores (MOS) from twenty human evaluators, nine Deep Convolutional Neural Network (DCNN) architectures were benchmarked and identified ResNet-101 as the most robust feature extractor. The features were mapped to MOS using regression models and compared with ten state-of-the-art NR-IQA algorithms. Experimental results demonstrated that PRIQA consistently outperforms existing methods, indicating its suitability as a practical quality control tool for identifying unreliable or low-quality parasite microscopy images and supporting more consistent downstream detection and diagnostic workflows in automated inspection systems.

## Full-text entities

- **Species:** Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

8 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12818675/full.md

## References

65 references — full list in the complete paper: https://tomesphere.com/paper/PMC12818675/full.md

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Source: https://tomesphere.com/paper/PMC12818675