# Sealing ability of Biodentine, zirconia reinforced glass ionomer cement and Mineral Trioxide Aggregate as furcation perforation repair materials: an in vitro analysis

**Authors:** Sumita Panwar, Yajuvender Singh Hada

PMC · DOI: 10.2340/biid.v13.45038 · Biomaterial Investigations in Dentistry · 2026-01-15

## TL;DR

This study compared how well three dental materials seal furcation perforations and found no significant differences between them.

## Contribution

The study provides new comparative evidence on the sealing ability of Biodentine, zirconia reinforced GIC, and MTA for furcation repair.

## Key findings

- Biodentine, zirconia reinforced GIC, and MTA showed similar sealing abilities.
- No statistically significant differences were found among the tested materials.
- All materials performed comparably under simulated clinical conditions.

## Abstract

The present study aimed to evaluate and compare the sealing ability of Biodentine, zirconia reinforced glass ionomer cement (GIC), and Mineral Trioxide Aggregate (MTA) as furcation repair materials.

A total of 50 extracted permanent maxillary molars were collected and divided into three experimental groups and one control group. Group I – Biodentine (n = 15), Group II – zirconia reinforced GIC (n = 15), Group III – MTA Angelus (n = 15), and unrepaired, control group (n = 5). Crowns of teeth in experimental groups were sectioned 3 mm above the cementoenamel junction and roots 3 mm below the furcation. Standardised endodontic access openings were prepared, canal orifices and root ends were sealed with sticky wax. After coating with nail varnish, a 1 mm furcation perforation was created at a standardised location using a round carbide bur. Samples were flushed, dried, and incubated at 37°C for 24 h to simulate clinical conditions. All samples were subjected to orthograde and retrograde methylene blue dye challenge followed by dye extraction with a concentration of 65% nitric acid. Samples were then analysed using 550 ultraviolet-visible spectrophotometers.

The results were analysed statistically by one-way analysis of variance (ANOVA) and Tukey’s multiple comparison tests.

No statistically significant difference in sealing ability was observed between Biodentine, zirconia reinforced GIC, and MTA when used as a furcation perforation repair material.

Within the limitations of this study, it can be concluded that Biodentine, zirconia reinforced GIC, and MTA showed sealing ability comparable to each other.

## Linked entities

- **Chemicals:** methylene blue (PubChem CID 4139), nitric acid (PubChem CID 944)

## Full-text entities

- **Chemicals:** MTA (MESH:C086631), methylene blue (MESH:D008751), nitric acid (MESH:D017942), Biodentine (MESH:C506393), MTA Angelus (-), zirconia (MESH:C028541)

## Full text

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## Figures

7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12817067/full.md

## References

33 references — full list in the complete paper: https://tomesphere.com/paper/PMC12817067/full.md

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Source: https://tomesphere.com/paper/PMC12817067