Metabolic consequences of perinatal bisphenol A and 17α-Ethinylestradiol exposure manifest in circadian alterations of energy homeostasis in adult male mice

TL;DR

Exposure to BPA or EE2 during pregnancy affects adult male mice's metabolism and circadian energy regulation.

Contribution

The study reveals distinct circadian metabolic disruptions in adult offspring due to perinatal exposure to BPA or EE2.

Findings

BPA-exposed offspring had reduced lean body mass, fat mass, and fasting glucose levels.

Both BPA and EE2 exposure altered circadian patterns of activity, food intake, and energy expenditure.

Effects were more pronounced during the night phase in exposed offspring.

Abstract

Environmental estrogenic chemicals can cross the maternal–fetal barrier and disrupt endocrine and metabolic regulation in the developing embryo/fetus. Bisphenol A (BPA) and 17α-ethinylestradiol (EE2) are widely present in the environment and have been linked to increased cardio-metabolic disease risk.

This study investigated the effects of maternal BPA and EE2 exposure on metabolic function and circadian energy regulation in male offspring.

Pregnant and lactating dams were chronically administered BPA (20 µg/kg bw/day) or EE2 (0.01 µg/kg bw/day) via osmotic minipumps from gestational day 9 to postnatal day 21 to mimic environmental exposure. Adult male offspring (60–80 days old) were assessed for body composition, fasting glucose, and metabolic and activity parameters using the TSE Phenomaster system.

BPA-exposed offspring exhibited reduced lean body mass, fat mass, fat ratio, and 24-hour fasting glucose levels compared to controls and EE2-exposed offspring. Both BPA- and EE2-exposed groups showed altered circadian patterns of locomotor activity, food intake, energy expenditure, and respiratory exchange ratio, with effects predominantly occurring during the night phase.

Maternal exposure to environmentally relevant doses of BPA or EE2 can alter the development and function of metabolic regulatory systems, producing distinct disruptions in circadian energy homeostasis in adult offspring. These differential effects likely reflect the partially overlapping yet distinct organizational and activational pathways through which these endocrine-disrupting chemicals act during the perinatal period.

Figures