A shoot endosymbiont colonizes pine host by unique and rhizobia-like mechanisms boosted by surface-fixed methanol

TL;DR

This study reveals how a methylotrophic bacterium colonizes pine trees by using methanol to enter and spread through plant tissues.

Contribution

The paper identifies unique and rhizobia-like colonization mechanisms of Methylorubrum extorquens in pine, including surface methanol utilization and tissue invasion routes.

Findings

Methylorubrum extorquens enters pine seedlings through the cylindrical sheath and epidermis.

Surface-bound methanol activates bacterial promoters for assimilation and tissue penetration.

Xylem serves as the main route for bacteria to reach the apical meristem in pine.

Abstract

Methylorubrum extorquens DSM13060 (Rhizobiales) has a specific capacity to live inside cells of bud meristems in pine trees. The bud niche is almost completely unstudied, although likely widespread in plants. It is unknown how the endosymbiotic methylotroph enters such crucial tissues of the plant. We hypothesized the bud colonization to occur mainly through the shoot epidermis enabled by host-produced methanol. We combined several microscopic methods to illustrate spatio-temporal colonization dynamics and methanol utilization by M. extorquens DSM13060 during the interaction. Our results showed that the endosymbiont mainly enters pine seedlings through cylindrical sheath, which is a layer of living cells surrounding primary root and transition zone. The cylindrical sheath played a central role in accumulation and proliferation of bacteria before entering deeper tissues. The endosymbiont also penetrated host through epidermis and stomatal apertures in stem and formed infection pocket-like structures upon entry. M. extorquens DSM13060 activated the mxaF-promoter on plant surfaces for methanol assimilation prior to shifting to the endosymbiotic lifestyle. Our results suggest that the surface-bound methanol was used for production of antioxidants that enable tissue penetration, documented earlier. Gradual cell-to-cell passage or formation of intracellular infection threads enabled the invasion past endodermis into the xylem. The xylem was observed to function as the main route to the apical meristem, where bacteria were present after 90 days of inoculation. Our study widens the previously known niches and reveals unique and rhizobia-like colonization mechanisms by the endosymbiont in the above and belowground parts of pine.

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