InSillyClo, a User-Friendly Web Application to Assist Large-Scale Golden Gate Cloning and MoClo Workflows

TL;DR

InSillyClo is a web tool that helps scientists streamline large-scale genetic cloning workflows using Golden Gate and MoClo methods.

Contribution

InSillyClo introduces a user-friendly, open-source web application for automating Golden Gate and MoClo cloning workflows.

Findings

InSillyClo automates the generation of workflow-related data for large-scale cloning.

The tool supports Modular Cloning with a versatile typing system for user-defined workflows.

InSillyClo is accessible both online and locally without requiring user registration.

Abstract

Systems and synthetic biology developments
often require the construction
of many variants of a genetic circuit of interest, resulting in large-scale
cloning campaigns. Golden Gate and Modular Cloning (MoClo), two powerful
technologies enabling the scale-up of cloning workflows, play a central
role for efficient circuit construction. These workflows include a
number of dry-lab tasks, which are time-consuming and error-prone
at scale. Currently, no software tool is available to handle these
tasks in a dedicated, time-saving, and user-friendly manner. We present
InSillyClo, an open-source web application to assist large-scale Golden
Gate cloning and MoClo workflows. It supports an easy specification
of genetic designs at any scale, followed by the automated generation
of comprehensive workflow-related data. Moreover, InSillyClo leverages
Modular Cloning with a versatile typing system of parts to generate
user-defined workflows. InSillyClo is open source, accessible with
or without user registration, and can also be used locally.

Figures