Integrated effects of pre-harvest high blue light and postharvest sodium nitroprusside on volatile oil composition and quality of cold-stored holy basil

TL;DR

This study shows that using high blue light during growth and sodium nitroprusside after harvest can reduce damage and improve quality in holy basil stored in cold conditions.

Contribution

The novel integration of high blue light and SNP treatment is shown to reduce oxidative stress and preserve volatile compounds in holy basil.

Findings

High blue light with 200 μM SNP reduced membrane damage and oxidative stress markers.

200 μM SNP preserved β-caryophyllene and humulene volatile compounds.

300 μM SNP increased antioxidant capacity under high blue light conditions.

Abstract

Holy basil (Ocimum tenuiflorum L.) is highly susceptible to chilling injury (CI) during postharvest storage, which limiting marketability and storage life. This study examined whether white or high-intensity blue light during cultivation, combined with postharvest sodium nitroprusside (SNP) treatment, alleviates CI and modulates volatile oil composition in green holy basil under cold storage. Plants were grown under white or high-intensity blue light and subsequently treated with 0, 100, 200, or 300 μM SNP before storage at 10 °C. Basil grown under high blue light and treated with 200 μM SNP showed the lowest electrolyte leakage and malondialdehyde levels, indicating reduced membrane damage and oxidative stress. High blue light also maintained higher antioxidant capacity and preserved key volatile compounds, including β-caryophyllene and humulene. Integrating high blue light with optimal SNP application effectively mitigates chilling-induced oxidative damage and improves postharvest quality, offering a practical strategy to extend shelf life in holy basil.

•High blue light during cultivation enhanced chilling tolerance in holy basil.•White and high blue light with 200 μM SNP showed the lowest malondialdehyde levels.•300 μM SNP increased antioxidant capacity under high blue light conditions.•β-caryophyllene and humulene were highest at 200 μM SNP treatment.

High blue light during cultivation enhanced chilling tolerance in holy basil.

White and high blue light with 200 μM SNP showed the lowest malondialdehyde levels.

300 μM SNP increased antioxidant capacity under high blue light conditions.

β-caryophyllene and humulene were highest at 200 μM SNP treatment.

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