Noninvasive Evaluation of the Rat Adenomyosis Model Constructed by Autologous Endometrial Implantation using Magnetic Resonance Imaging

TL;DR

This study shows that MRI can noninvasively evaluate a rat model of adenomyosis, a condition where endometrial tissue grows into the uterine muscle.

Contribution

The study introduces a noninvasive MRI method to evaluate a rat adenomyosis model and visualizes the junctional zone for the first time in this context.

Findings

MRI successfully detected adenomyosis lesions in all model group rats, showing myometrial thickening and T2 hypersignal spots.

Histological analysis confirmed MRI findings, showing hyperplasia and compact cell arrangement in the junctional zone.

MRI can distinguish between ectopic endometrial tissue and muscle hyperplasia based on signal intensity patterns.

Abstract

Dynamic changes in adenomyotic lesions in animal models have been difficult to observe and evaluate in vivo on a regular basis. Therefore, this study aims to investigate the feasibility of establishing a rat model of adenomyosis through autologous endometrial implantation and to assess the value of magnetic resonance imaging (MRI) for noninvasive evaluation of the model.

Forty rats were randomly divided into two groups (20 rats in the control group, 20 rats in the model group). A rat adenomyosis model was constructed through autologous endometrial implantation. Three months after the modeling surgery, the rats underwent MRI examination, including T2-weighted axial imaging and T1-weighted axial imaging. The thickness of the uterine myometrium and junctional zone was measured. Following the MRI, the rat uterus was sliced for hematoxylin-eosin (HE) staining.

In the model group, lesions of adenomyosis were successfully established in all surviving rats. The myometrium of the rat uterus showed uneven thickening accompanied by scattered spotty T2 hypersignal. The junctional zone appeared as a low-signal band between the endometrium with high signal and the myometrium. The average thicknesses of both the myometrium and the junctional zone were significantly greater in the model group compared to the control group, with the differences reaching statistical significance.

Ectopic endometrium can lead to hyperplasia of the peripheral muscle cells in the myometrium, which is manifested on T2-weighted images as localized thickening and hypo-intensity of the myometrium interspersed with punctiform hyperintensity. Histologically, regions of low signal intensity refer to hyperplasia of smooth muscle, while bright foci on T2-weighted images correspond to ectopic endometrial tissue and cystic dilation of glands. This study proved the noninvasive evaluation of a rat adenomyosis model and described the junctional zone in rats using MRI techniques. Histological examination using HE staining confirmed a higher nuclear-to-cytoplasmic ratio and a more compact cell arrangement in the junctional zone region of rats compared to the outer myometrium, which could explain its hypointensity.

MRI is a valuable method for evaluating the rat adenomyosis model non-invasively. Furthermore, the successful visualization of the junctional zone in the rat uterus using MRI may have potential applications in further evaluating the progression of adenomyosis.

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