The Size of Human Mesenchymal Tissue Stem Cell Turnover Units is a Major Determinant for Maintaining High Stem Cell Fractions With Serial Culture Expansion
James L. Sherley, Chen Cao, Celia Sommer, Alex Dahlkemper, James Sugai, Hitesh Chopra, Darnell Kaigler
TL;DR
This paper shows that the size of stem cell turnover units affects how well mesenchymal stem cells maintain their numbers during repeated lab culture.
Contribution
The study identifies smaller stem cell turnover units as a key factor in maintaining stable stem cell fractions during serial culture expansion.
Findings
Stem cell fraction stability is higher in samples with smaller turnover units.
Smaller turnover units reduce stem cell dilution during expansion.
This mechanism is likely applicable to other human tissue stem cells.
Abstract
Recently, we used kinetic stem cell (KSC) counting to show that the stability of the stem cell fraction (SCF) during serial culture expansion of human oral alveolar bone mesenchymal tissue cell (MTC) preparations varied significantly among patient donors. Whereas some patient donor samples’ SCFs declined rapidly, others showed a moderate decrease; and still others had highly stable SCFs during serial culture expansion. Defining the cell kinetics basis for these differences in SCF stability could lead to effective solutions for the problem of loss of mesenchymal stem cell (MSC) function when MTC preparations are expanded for research and clinical applications. Using KSC counting, we show that greater SCF stability is associated with smaller stem cell turnover units, which reduce the SCF by cell dilution. This finding confirms earlier evidence and proposals that, by limiting the…
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Taxonomy
TopicsMesenchymal stem cell research · Cancer Cells and Metastasis · Hematopoietic Stem Cell Transplantation