# OUTPOST: A comprehensive analysis software for whole‐metagenome shotgun sequencing incorporating group stratification

## TL;DR

OUTPOST is a new software for analyzing whole-metagenome shotgun sequencing data that handles multiple groups and offers more comprehensive features than existing tools.

## Contribution

OUTPOST introduces new methods for multi-group analysis and biomarker identification in metagenomic studies.

## Key findings

- OUTPOST includes 14 modules and over 50 functions for metagenomic analysis.
- It outperforms 17 existing tools in comprehensiveness and multi-group experimental design capabilities.

## Abstract

The whole metagenOme shotgun seqUencing sTream Pipeline that is cOmprehensive and uSeful for mulTi groups experiments (OUTPOST) is a comprehensive analysis software for whole‐metagenome shotgun sequencing incorporating group stratification, which encompasses 14 modules and boasts over 50 functions, distinguishing itself for its comprehensiveness when compared with 17 existing whole‐metagenome shotgun sequencing (WMGS) tools. OUTPOST introduces innovative methods for multi‐group experimental designs and meta‐analysis‐based biomarker identification.