# A minimal RNA-cleaving DNAzyme and its catalytic mechanism

## TL;DR

Researchers designed a minimal DNAzyme that can cleave RNA and revealed its catalytic mechanism involving zinc ions and specific base pairing.

## Contribution

A minimal RNA-cleaving DNAzyme with a novel catalytic mechanism elucidated through structural analysis.

## Key findings

- The DNAzyme has a catalytic core of two nucleotides and a substrate core of three nucleotides.
- The DNAzyme's structure is B-DNA-like with a non-Watson–Crick base pair in the catalytic loop.
- Zn2+ coordinates with a G-base to facilitate RNA cleavage via a proposed acid-base mechanism.

## Abstract

Although natural sources of enzymes are limited to protein and RNA, some artificial DNAs exhibit catalytic activities. Representative functions of such DNAs, i.e. DNAzymes, are cleavage and ligation of nucleic acids. Here we developed a minimal DNAzyme with an RNA-cleaving activity by in vitro selection and secondary structure-based design. Its catalytic and substrate cores are only two and three nucleotides, respectively. This DNAzyme showed strict Zn2+ dependence at optimal pH 7.0–7.5. To elucidate its catalytic mechanism, we determined its three-dimensional structure by X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. The results consistently showed a B-DNA-like structure with base pairing and stacking throughout the molecule, unlike the kinked structures of larger DNAzymes. Notably, an A-base in the catalytic loop and a G-base in the substrate loop formed a non-Watson–Crick base pair. The catalytic Zn2+ coordinates to N7 of that G-base, enabling the Zn2+-hydrated water molecules to contacts O2′ and O5′ at the cleavage site. Considering that Zn(OH)+ and Zn2+ co-exist at the enzyme’s optimal pH, we propose a catalytic mechanism whereby these ions act as the base withdrawing H+ from O2′ and the acid donating H+ to O5′, generating the cleaved ends with 2′,3′-cyclic phosphate and OH groups.

Graphical Abstract