# Productivity-driven decoupling of microbial carbon use efficiency and respiration across global soils

## TL;DR

Microbial efficiency in using carbon in soils varies with ecosystem productivity, limiting carbon storage in productive regions.

## Contribution

A stoichiometry-based approach reveals a nonlinear relationship between microbial C use efficiency and respiration across ecosystems.

## Key findings

- In low-productivity regions, microbial C use efficiency declines with increasing respiration.
- In productive regions, C use efficiency stabilizes at low levels despite higher respiration.
- Microbial trade-offs between assimilation and homeostasis limit soil carbon storage in productive ecosystems.

## Abstract

Despite extensive research on soil microbial carbon (C) use efficiency (CUE), its linkage to actual soil C storage remains ambiguous. A key uncertainty is that CUE estimates from short-term labeling incubations assume a linear negative relationship with respiration rates, overlooking nonlinear interactions and long-term microbial acclimation. Here, we use a stoichiometry-based approach to estimate CUE (CUEST), which links soil resource availability to microbial demand and captures microbial adaptability under resource constraints. We synthesized 1094 paired observations of CUEST and heterotrophic respiration rate (Rh) across natural ecosystems and found a nonlinear relationship between them governed by ecosystem productivity. In low-productivity arid and cold regions, CUEST declined with increasing Rh, whereas in productive tropical and temperate regions, CUEST stabilized at a low level (0.27 ± 0.11) as Rh exceeded 340 ± 10.8 grams of C per square meter per year. This shift reflects microbial trade-offs between C assimilation and stoichiometric homeostasis, revealing a decoupling of microbial growth from respiration that limits the capacity of productive ecosystems to store additional soil C.

Resource-dependent microbial adaptability decouples microbial growth from respiration and limits soil carbon storage.