# A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot, immunoprecipitation, and immunofluorescence

## TL;DR

This paper evaluates 24 commercial antibodies for GCase to help researchers choose reliable ones for common lab techniques.

## Contribution

The study provides a standardized evaluation of GCase antibodies across multiple applications to improve reproducibility.

## Key findings

- Twenty-four GCase antibodies were tested for western blot, immunoprecipitation, and immunofluorescence.
- Results are shared as an open resource to help researchers select high-performing antibodies.
- The study aims to address antibody reproducibility issues in scientific research.

## Abstract

The human
GBA1 gene encodes glucocerebrosidase (GCase), a lysosomal enzyme that hydrolyzes glucosylceramides. Variants in
GBA1 and reduced GCase activity have been linked to Parkinson’s disease and Gaucher’s disease. Here we have characterized twenty-four GCase commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.