# Dietary Fat Intake Attenuates Vitamin A Deficiency-Associated Elastic Fiber Remodeling and Lipid Reduction in the Alveolar Niche in Mice

## TL;DR

A diet high in fat reduces the negative effects of vitamin A deficiency on lung structure and lipid levels in mice.

## Contribution

The study reveals that high-fat diets can mitigate vitamin A deficiency-induced changes in lung elastic fibers and lipid droplets in mice.

## Key findings

- Vitamin A deficiency caused dense elastic fiber packing and increased fibrillin expression in lean mice.
- High-fat diets reduced alveolar lipid droplets and lung airspace volume compared to control diets.
- Combining high-fat diets with vitamin A deficiency attenuated the negative effects on lung structure.

## Abstract

Vitamin A deficiency (VAD) and obesity are widespread nutrition-related health conditions that are independently associated with pulmonary remodeling processes linked to lung function decline and respiratory diseases.

This study tested the hypothesis that VAD-related pulmonary alterations are aggravated by diet-induced obesity.

Eight-week-old C57BL/6J/129Sv mice with a deletion of lecithin-retinol-acyltransferase (Lrat−; impaired vitamin A storage) were fed vitamin A deficient control diet (CD, n = 13) or high-fat diet (HFD, n = 15) to induce VAD in lean (CD-VAD, n = 13) or obese (HFD-VAD, n = 13) mice. Wild-type mice receiving vitamin A-containing CD or HFD served as controls. After 20 wk, lungs were subjected to structural and molecular analyses by stereology, western blot, and high-pressure liquid chromatography-mass spectrometry. Statistics used were 2-way analysis of variance.

Pulmonary vitamin A reserves were efficiently depleted in CD-VAD and HFD-VAD (P < 0.001 compared with controls). In CD-VAD, 76% of pulmonary elastic fibers appeared densely packed (CD: 53%, P < 0.01), and expression of fibrillin was 110% higher compared with CD (P < 0.01), indicating a higher septal microfibril content. Elastin expression was slightly reduced in HFD-groups (HFD: 14%, HFD-VAD: 16% of respective controls, both P < 0.05), whereas neither diet nor VAD affected expression levels of collagen I or III. Lipid droplet volumes decreased by 32% in septal fibroblasts (P < 0.05) and by 53% in alveolar epithelial type 2 (AE2) cells in CD-VAD, compared with CD. HFD alone led to a 20% reduction in lung airspace volume, a 13% decrease in septal surface area, and a 15% reduction in AE2 cell numbers compared with CD. These VAD- and obesity-related changes were alleviated or absent in HFD-VAD.

VAD-induced elastic fiber remodeling and lipid droplet reduction in the alveolar region of lean mice, whereas HFD resulted in smaller lungs containing less AE2 cells. Both VAD- and obesity-related effects were attenuated in HFD-VAD, indicating mutually mitigating effects.

Image 1