Corrigendum: Rhodococcus parequi sp. nov., a new species isolated from equine farm soil closely related to the pathogen Rhodococcus equi
José A. Vázquez-Boland, Jorge Val-Calvo, Fabien Duquesne, Francesca Decorosi, Carlo Viti, Sandrine Petry, Mariela Scortti
Abstract
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsInfectious Disease Case Reports and Treatments · Infections and bacterial resistance · Bacterial Genetics and Biotechnology
In the originally published version of this article, an error was identified in the sequences of the universal primers 27F and 1492R reported in the first paragraph of the section ‘16S rRNA Gene Sequence’. The correct oligonucleotide primer sequences are as follows:
27F: 5′-AGAGTTTGATCCTGGCTCAG-3′ (20 nt)
1492R: 5′-CGGCTACCTTGTTACGACTT-3′ (20 nt)
The sequences of the oligonucleotide primers are based on those reported by Lane [1] except that our version of primer 1492R is two nucleotides shorter at the 5′ end (as often used by others). Additionally, both primers were optimised for R. equi and R. parequi by including the nucleotide C at the ‘M’ (C/A) position in 27F, and at the ‘Y’ (C/T) position in 1492R.
The authors apologise for any confusion or inconvenience caused.
The reference list from the paper itself. Each links out to its DOI / PubMed record.
- 1Lane D 16S/23S r RNA sequencing Stackebrandt E Goodfellow M Nucleic Acid Techniques in Bacterial Systematics Chichester Wiley 1991115175