# Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats

## TL;DR

Melatonin protects rat stomach lining from damage caused by chronic low oxygen levels by reducing oxidative stress and preventing cell death.

## Contribution

This study identifies melatonin as a protective agent against hypoxia-induced gastric injury via modulation of oxidative stress and JNK signaling.

## Key findings

- Chronic intermittent hypoxia causes gastric damage through oxidative stress and JNK-mediated apoptosis.
- Melatonin treatment reduces oxidative stress markers and prevents apoptosis in hypoxia-exposed rats.
- Melatonin suppresses the JNK-Bax/Bcl-2 pathway, protecting gastric mucosa.

## Abstract

To investigate the mechanism of chronic intermittent hypoxia on gastric injury in rats and the intervening effect and possible mechanism of melatonin.

Forty-eight male Wistar rats were randomly divided into normal control, intermittent hypoxia, and melatonin treatment groups. Subgroups (n = 4 per time point) were treated for 2, 4, 6, and 8 weeks. Gastric tissue morphology, gastric juice pH, pepsin levels, oxidative stress markers (MDA and SOD), and the expression of JNK and apoptosis-related genes (Bax, Bcl-2) were assessed.

The intermittent hypoxia group exhibited significant gastric mucosal damage, decreased pH, increased pepsin, elevated MDA, reduced SOD, and upregulation of JNK and Bax/Bcl-2 mRNA ratio. Melatonin treatment markedly alleviated these pathological and molecular changes compared to the intermittent hypoxia group (P < 0.05).

Chronic intermittent hypoxia induces gastric mucosal injury, which is associated with oxidative stress imbalance and activation of JNK-mediated apoptotic signaling. Melatonin exerts a protective effect by enhancing antioxidant capacity and suppressing the JNK-Bax/Bcl-2 pathway.