# Draft genome of a member of ascomycotal fungal genus Pseudopithomyces (family Didymosphaeriaceae)

**Authors:** Krithika Arumugam, Sherilyn Ho, Irina Bessarab, Falicia Q. Y. Goh, Mindia A. S. Haryono, Ezequiel Santillan, Stefan Wuertz, Yvonne Chow, Rohan B. H. Williams

PMC · DOI: 10.1128/mra.00173-23 · 2025-11-25

## TL;DR

This paper presents the draft genome of a Pseudopithomyces fungus isolated from brewery wastewater, providing insights into its genetic makeup and taxonomy.

## Contribution

The study provides a high-quality draft genome for a Pseudopithomyces species using multiple taxonomic validation methods.

## Key findings

- The draft genome is 39.65 Mbp long and consists of 43 contigs.
- BUSCO analysis showed a completeness of 98.9%, indicating high quality.
- Taxonomic classification was confirmed using ITS, mitochondrial DNA, and k-mer analysis.

## Abstract

We report a draft genome of a species from the ascomycotal fungal genus Pseudopithomyces (isolate name SBW1) obtained using a culture isolate from brewery wastewater. The 43 contig genome has a length of 39.65 Mbp, with Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 98.9%. Taxonomic assignment was supported by internal transcribed spacer (ITS) sequence, mitochondrial DNA sequence, and k-mer analysis.

## Linked entities

- **Species:** Pseudopithomyces (taxon 1892769)

## Full-text entities

- **Species:** Pseudopithomyces (genus) [taxon 1892769]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12798004/full.md

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Source: https://tomesphere.com/paper/PMC12798004