# The whole-genome sequence of the halotolerant nitrogen-fixing bacterium Azotobacter salinestris strain AZT-41

**Authors:** Lekhana S. M., Pushya Pradeep, Deepesh Nagarajan, Hanumantharaju K. N., Sreenivasa M. Y., Chennappa Gurikar

PMC · DOI: 10.1128/mra.00695-25 · 2025-12-09

## TL;DR

This paper presents the whole-genome sequence of Azotobacter salinestris, a salt-tolerant nitrogen-fixing bacterium useful for sustainable agriculture.

## Contribution

The study provides the first whole-genome sequence of Azotobacter salinestris strain AZT-41, revealing its potential for nitrogen fixation and soil improvement.

## Key findings

- The genome is 5.29 Mb with 4,423 predicted genes, including nitrogen fixation (nif) genes.
- The bacterium shows potential for enhancing soil fertility and bioremediation in saline soils.
- It offers eco-friendly solutions for sustainable agriculture through biocontrol and nitrogen fixation.

## Abstract

Azotobacter salinestris, a diazotrophic, nitrogen-fixing bacterium, promotes plant growth and bioremediation in saline soils. Whole-genome sequencing (5.29 Mb, 30× coverage, N50: 4840) identified 4,423 genes, including nif genes. This study highlights its genomic potential for sustainable agriculture, offering eco-friendly solutions through enhanced nitrogen fixation, soil fertility improvement, and biocontrol applications.

## Linked entities

- **Genes:** S100A8 (S100 calcium binding protein A8) [NCBI Gene 6279]
- **Species:** Azotobacter salinestris (taxon 69964)

## Full-text entities

- **Chemicals:** nitrogen (MESH:D009584)
- **Species:** Azotobacter salinestris (species) [taxon 69964]
- **Cell lines:** AZT-41 — Mus musculus (Mouse), Mouse kidney carcinoma, Cancer cell line (CVCL_0151)

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Source: https://tomesphere.com/paper/PMC12797993