# Bacteriome and resistome dysbiosis in subclinical mastitis and antibiotic-treated milk of dairy cows

## TL;DR

This study shows how bacteria and antibiotic resistance change in cow milk during mastitis and after antibiotic treatment, suggesting better methods to ensure milk safety and reduce resistance.

## Contribution

The study identifies distinct microbial and resistance profiles in subclinical mastitis and antibiotic-treated milk using shotgun metagenomics.

## Key findings

- Milk from cows with subclinical mastitis and those treated with antibiotics showed unique bacteriome profiles.
- Resistance gene repertoires were enriched in diseased and treated milk samples.
- The findings emphasize the need for improved diagnostics and alternative treatments to combat antimicrobial resistance.

## Abstract

Shotgun metagenomics revealed distinct bacteriome profiles in subclinical mastitis, antibiotic-treated, and healthy cow milk, with enriched resistance repertoires in diseased and treated samples. Findings highlighted the need for better diagnostics, precision antimicrobial use, and antibiotic alternatives to ensure milk safety and address antimicrobial resistance in dairy farming.